Figure 4: Speckle-tracking echocardiography, measurement of longitudinal strain (a) The endocardial border is manually traced in the end-systolic frame. The automated software creates a region-of-interest that includes the entire myocardial thickness. (b) The operator is prompted to review and approve the adequacy of tracking for each segment (c) The final strain curves. Color-coded curves depict segmental strain whereas the dotted white curve depicts the average strain. The lower left corner shows the parametric image, displaying the timing and the magnitude of segmental longitudinal strain.